gp120-induced Vγ2Vδ2 T cell death depends on p38-caspase pathway. (A) Vγ2Vδ2 T cells were untreated or pretreated with specific caspase inhibitors (2μM) for 1 hour at 37°C before incubated with CN54 gp120 (10 μg/mL) for 24 hours. (B) Vγ2Vδ2 T cells were incubated with or without Bal or CN54 gp120 protein for 10 hours. Cells were then collected for Western blot assay with specific antibodies. (C) Vγ2Vδ2 T cells were untreated or pretreated with specific inhibitors for 1 hour at 37°C before incubating with CN54 gp120 for 24 hours. (D) Vγ2Vδ2 T cells were untreated or pretreated with SB203580 or ZVAD for 1 hour at 37°C before incubated with CN54 gp120 for 10 hours. Cells were collected for Western blot assay with specific antibodies. Inhibition of cell death was calculated in relation to gp120-induced cell death with untreated Vγ2Vδ2 T cells. Data are representative of 3 independent experiments (error bars, SD).