SHP2 knockdown down-regulates STAT5, ERK1/2, and AKT signaling and MCL1 and BCLXL levels. CD34⁺DsRED⁺ cells were expanded in culture for 7 days, and protein expression was evaluated at different time points. (A) Western blots showing expression of SHP2, total and phosphorylated ERK, AKT, JAK2, STAT3, and STAT5 at day 2. (B) Western blots showing expression of SHP2, total and phosphorylated ERK, AKT, JAK2, STAT3, and STAT5 at day 7. Results shown are representative of 3 experiments. (C) Western blots showing expression of the antiapoptotic genes BCL2, BCLXL, and MCL1 at day 7. (D) Phosphorylated STAT5 levels measured by flow cytometry in CD34⁺-expressing cells at day 3 and day 5.