AMD3100 leads to rapid and significant mobilization of B- and T-cell subpopulations as well as NKs and pDCs into the peripheral blood. Data were collected at baseline (before mobilization) and 2, 4, and 6 hours after treatment with AMD3100 and are expressed as the fold change in cell number in the peripheral blood compared with baseline. Red squares represent mobilization with G-CSF alone (n = 6); blue triangles, mobilization with AMD3100 alone (n = 6); and green circles, mobilization with G-CSF + AMD3100 (n = 6). Error bars represent SEM. The data were pooled from 2 separate experiments. In each experiment, groups of 3 animals each were treated with AMD3100, G-CSF, or G-CSF + AMD3100, and data at baseline and at 2, 4, and 6 hours after mobilization were log-transformed and then subjected to ANOVA and posthoc Tukey testing. ANOVA statistics comparing all 3 treatment groups are shown below the x-axis. Posthoc Tukey statistics comparing AMD3100 with G-CSF (blue symbols), G-CSF + AMD3100 with G-CSF alone (green symbols), or G-CSF + AMD3100 with AMD3100 (black brackets) are shown for each time point. ns indicates not significant (P > .1). ^P < .1. *P < .05. **P < .01. ***P < .001.