Methylation of MIR34B/C. (A) U-MSP showed that the methylated control PC was totally methylated, and all 8 normal controls (N1-N8) were unmethylated. In the M-MSP, the methylated control was methylated, but all normal controls were totally unmethylated. (B) For the cell lines, KMS-12-PE, LP-1, and NCI-H929 were completely methylated, MOLP-8, OPM-2, and WL-2 were hemizygously methylated, but RPMI-8226 and U-266 were unmethylated of MIR34B/C. (C) Methylation of MIR34B/C in primary myeloma marrow samples at diagnosis (Dx) and (D) at relapse/progression (R). (E) Sequence analysis of M-MSP product from bisulfite-treated relapsed myeloma DNA showed that the cytosine (C) residues of CpG dinucleotides were methylated and remained unchanged, whereas the other C residues were unmethylated and were converted to thymidine (T).