BAD-LAMP mRNA expression profile in human leukocytes. (A) Gene microarray quantitation of BAD-LAMP mRNA expression in human leukocytes. Results are shown as the fluorescent signal intensity for Affymetrix Human Genome U133 PLUS 2.0 ProbeSet 219463_at (expressed in arbitrary units in log scale). Quality controls, data sources, and data normalization have been described previously.32 Neu indicates neutrophils; pMθ, PBMC-derived macrophages; Mo-Mθ, monocyte-derived macrophages; Mo-DC, monocyte-derived GM-CSF + IL-4 DCs; CD16 DC, blood Lin−HLA−DR+CD16+ DCs; BDCA1 DC, blood BDCA-1+ DCs; BDCA3 DC, blood BDCA-3+ DCs; BL, blood B lymphocytes; CD4 TL, blood CD4+ T lymphocytes; CD8 TL, blood CD8+ T lymphocytes; (B) Tissue expression of BAD-LAMP assessed by northern blot. A signal was detected only in the adult human brain. Actin mRNA levels are shown as a control. (C) Detection of BAD-LAMP transcript in human spleen RNA total extracts by nested RT-PCR. (D) Detection of BAD-LAMP transcript in total RNA extracts from human samples. Actin levels after the first PCR round are shown as a control. A plasmid containing BAD-LAMP cDNA was used as a positive control.
