T_MH60 mediate GVL against mBC-CML. B6.H60 mice were irradiated and reconstituted with C3H.SW BM, B6.H60 mBC-CML cells with no T cells, or with 3 × 10⁵ or 5 × 10⁴ CD8⁺CD44⁺ cells from DEC-H60–vaccinated or unmanipulated C3H.SW mice or 5 × 10⁴ CD8⁺CD44⁻CD62L⁺ T_N from unmanipulated C3H.SW mice. (A) Survival. All deaths were from leukemia. P < .0001 comparing BM alone with any T_MH60 group; P = .0124 comparing BM alone with T_N recipients; P > .2 comparing BM alone with any T_M group. P ≤ .026 comparing any T_MH60 group with any T_M or T_N group. (B) Number of EGFP⁺NGFR⁺ cells in peripheral blood at day 14. P < .05 comparing either T_MH60 recipient group with its T_M control; P < .008 comparing any T_MH60 recipient group with the BM alone control. Tet^H60+ cells accumulated only in T_MH60 recipients. (C) Representative flow cytometry of peripheral blood at day 9. (D) Quantitation at days 9, 14, 20, and 29. Each symbol represents data from an individual animal; solid lines are mean values. Data are pooled from 3 independent experiments with similar results.