Protein localization of PML and its fusion proteins in soluble and/or insoluble fractions with or without As₂O₃. (A) HA-PML was overexpressed in HeLa cells with or without As₂O₃ (10μM for 2 hours), and the whole-cell lysate (soluble) and pellets (insoluble fraction) were obtained for immunoblotting. Black and white arrowheads indicate modified/oligomerized and degraded PML, respectively. (B) The same assay described in panel A was performed using PR-WT, PR-B/L-mut, and the long form of PML-RARA (PR-long). White circles indicate PR-WT or PR-B/L-mut and white squares indicate PR-long protein. Black and white arrowheads indicate modified/oligomerized and degraded fusion proteins, respectively. Note that neither protein transportation from the soluble to insoluble fraction (lanes 7 vs 8 and 13 vs 14) nor modified/oligomerized or degraded proteins after treatment with As₂O₃ (lanes 8 and 14) were observed with PR-B/L-mut. (C) Protein expression levels in panel B were measured using BioMax 1D software, and the relative intensity is depicted as a bar graph.