Analyses of the protein localization of PR-L and PR-L-B2-mut2 using IF staining. (A) Flag-tagged PR-L was overexpressed in HeLa cells with or without As₂O₃. Anti-FLAG and PML antibodies were used to detect PR-L and endogenous PML, respectively. PML bodies were confirmed in the nucleus with a microgranular pattern without As₂O₃ (i,ii, and iv) and a macrogranular pattern with As₂O₃ (v,vi, and viii). (B) A similar assay was performed using PR-L-B2-mut2. Note that the localization of PR-L-B2-mut2 was confirmed in the nucleus as a diffuse pattern with or without As₂O₃.