Sequence comparison of laminin α1, osteopontin, and VEGFA in various species and overview diagram of the loops where angiogenic peptides were grafted into MCoTI-II and SFTI-1 scaffolds. (A) Sequence alignment of the laminin α1, osteopontin, and QK sequences from different organisms. Active angiogenesis sequences are highlighted in gray. Nonconserved residues in the active region are bolded. The asterisk indicates that the QK peptide is a chemically designed peptide that mimics the sequence of VEGFA. (B) MCoTI-II (PDB ID: 1HA9) and SFTI-1 (PDB ID: 1JBL) used as scaffolds for grafting 3 major angiogenic sequences. Loop 6 of MCoTI-II and the trypsin-inhibitor loop of SFTI-1 are the regions for introducing pro-angiogenic sequences. These loops are circled in black.