CAM assay results. (A) Comparison of blood vessel count of the linear and MCoTI-II–grafted peptides. (B) Comparison of blood vessel count of the linear and SFTI-1–grafted peptides. (C) Comparison of blood vessel count with a range of concentrations of linear OPN and SFTI-OPN. Comparison of blood vessel count of the linear OPN and SFTI-OPN. Each group of test and control peptides are n = 12 except VEGF, which is n = 9, and SFTI-OPN 0.5 μg/mL, which is n = 6. Linear angiogenic sequences were grouped as “a” and all grafted peptides were grouped as “b.” Different batches of C cortunix eggs were used for this assay. The following peptide concentrations were tested: VEGF: 0.26nM; linear LAM: 81.2μM; linear OPN: 63.1μM; linear OPN: 32μM; linear OPN 3.2μM; linear QK: 39.4μM; MCoTI-II: 14.5μM; MCo-LAM: 14.6μM; MCo-OPN: 13.9μM; SFTI-1: 33μM; SFTI-LAM: 36.4μM; SFTI-LAM: 3.6μM; SFTI-OPN: 32μM; SFTI-OPN: 3.2μM; SFTI-OPN: 320nM; and SFTI-QK: 24.6μM. All means are significantly different. *P ≤ .05 versus control (medium). Error bars indicate SEM. A red line in each of the bar graph indicates the baseline for a peptide to be considered as having angiogenic activity.