Changes in gene expression in LBW-ECFCs. PCR arrays for angiogenesis genes were performed as described in “Methods.” Data represent mean changes of experiments using 3 independent < 1500-g LBW-ECFCs and > 2700-g CT-ECFCs. (A) Volcano plot of angiogenesis PCR array. The x-axis indicates the fold change in Log2 scale; and the y-axis, P value in −Log10 P scale. Using a 2-fold change and a P value of .05 as the cutoff threshold, 11 genes from the left region and 15 from the right region were selected. Red represents high level of expression; green, low level of expression; and black, no change. (B) Representative experiments of THBS1 Western blot in 3 independent < 1500-g LBW-ECFCs or > 2700-g CT-ECFCs. Cell lysate (40 μg) was resolved on 4% to 12% SDS-PAGE gradient under reducing conditions. The blots were probed with anti-THBS1 monoclonal antibody or anti–β-tubulin as loading control. Numbers represent a relative quantification performed by determining the ratio THBS1/tubulin intensities. Bars represent mean ± SEM of the relative intensities. *P < .05.