Enhanced long-term reconstitution capacity of LT-HSCs from Dmtf1^−/− mice. Recipient mice were transplanted with 2 × 10⁵ BM-MNCs from WT or KO mice (CD45.2⁺) and an equal number of BM-MNCs from BJ mice (CD45.1⁺; A) or 100 CD34-135-KSL cells from Dmtf1^+/+ or Dmtf1^−/− mice plus 5 × 10⁵ competitor MNCs from BJ mice to reconstitute lethally irradiated B6/BJ-F1 recipients (CD45.2 ⁺ 45.1⁺; B). Chimerism was assessed monthly. Data shown are the mean chimerism shown as percentage ± SD of donor-derived cells in PB (n = 4 per group per experiment). **P < .05. Next to panels A and B in bar graphs are the results of secondary transplants in which each secondary recipient received 2 × 10⁵ BM-MNCs from a primary recipient 4 months after the primary transplantation. Data analysis in secondary recipients was performed 4 months after transplantation. (C) One representative dot plot of CD48/150 distribution in the KSL fraction of primary recipient BM at 4 months after transplantation with LT-HSCs from KO or WT mice.