Dmtf1^−/− BM cells have enhanced rebound kinetics after myelotoxic stress. (A) Kinetics of PB count during recovery from BM suppression after a single injection of 5-FU (150 mg/kg; n = 7-8 per genotype). (B) BM cells from WT or Dmtf1-KO mice were collected 5 days after a single injection of 5-FU (150 mg/kg) and were stained with Hst/Py. One representative set of dot plots from 3 independent experiments with similar results showing cell-cycle status of WT and KO KSL cells. (C) BrdU incorporation in slamKSL cells at day 5 after a single injection of 5-FU (150 mg/kg). Results are shown as average ± SD (n = 3). *P < .03. (D) Dmtf1^+/+ or Dmtf1^−/− BM cells were transplanted into irradiated B/J mice (n = 10 for each group). After 3 months, 5-FU (135 mg/kg) was injected IP weekly for 3 consecutive weeks and survival was monitored daily. Data shown were plotted in a Kaplan-Meyer curve and the results were analyzed with a log-rank nonparametric test.