Potassium levels do not change and calcium levels decrease after transfusions of older RBCs. The mean ± SEM for serum levels of (A) potassium, (B) total calcium, and (C) corrected calcium calculated as [(0.8 × (4.0 − subject's albumin)) + serum calcium]. The vertical arrow indicates the pretransfusion time point; and dotted lines, the reference ranges. The P values are as specified in the figure comparing the paired area under the curve of the mean of the outcome parameter for the N = 14 volunteers from 0 to 24 hours after the fresh and older RBC transfusions.