CFU assays of SALL4-transduced cells. (A) CFU progenitors are GFP-positive. HSC cells selected for CFU assays were GFP-positive, which verified that the cells were successfully transduced and overexpressing SALL4 (10×). (B) Various CFU colonies are able to differentiate from SALL4-induced HSCs. Aged SALL4-induced HSCs were plated in MethoCult and observed for CFU colonies. Numerous lineages were observed in CFU assays using the SALL4-induced HSCs, including CFU-GEMM, BFU-E, and CFU-GM colonies (10×). These data demonstrated that the aged HSCs transduced with SALL4 were capable of differentiating into different blood cell lineages. (C) Number of CFU colonies formed from SALL4-induced hematopoietic stem cells. The number of CFU colonies was counted 13-18 days after SALL4-induced or GFP-induced cells were cultured in CFU MethoCult media. The representative data from day 18 are shown. (D) Types of CFU colonies formed at day 18.