αβNKT cells are not selected against functional TCRγ chains. Sorted NKT and CD4⁺ thymocytes were retrovirally transduced with the indicated constructs and stained with anti–γδTCR Ab. (A) Representative results of the FACS analysis used to determine the percentage of γδTCR⁺ cells among the GFP⁺ cells (B). (B) Summarized results from 4 independent experiments. The P values of a paired t test are shown. (C-D) Percentages of TCRVγ4⁺TCRγδ⁺ and TCRVγ4⁻TCRγδ⁺ among GFP⁺ cells from WT or TCRδ-deficient (KO) mice measured on day 2 (C) and day 3 after infection.