Transplanted HSPCs home preferentially to the trabecular-rich metaphysis. (A) Spatial distribution map of individual HSPCs within the femur 15 hours after transplantation. The map was constructed by transposing the spatial distribution of individual HSPCs (○) from 5 alternate serial sections onto a representative section taken from the beginning of the series. Toluidine blue stain. Scale bar represents 1 mm. Micrograph captured using a 10× (UPLSApo NA 0.4) objective on an Olympus BX51 microscope fitted with a SPOT RT-SE6 camera. Montage constructed with Photoshop CS2. (B) The spatial distribution of individual HSPCs from longitudinal sections of femur demonstrates a preferential distribution of HSPCs to the trabecular-rich metaphysis. Each symbol represents an individual recipient. Experimental repeats more than or equal to 2. Data are mean ± SEM.