DC accumulation and spontaneous activation of T cells in perforin^−/−DC-Fas^−/− mice. (A) Flow cytometry of CD11c^highI-A^b+ DCs and CD11c^lowPDCA-1⁺ pDCs in 4-week-old WT, perforin^−/−, DC-Fas^−/−, and perforin^−/−DC-Fas^−/− mice. (B) Staining of I-A^b, CD80, CD86, and ICAM-1 on CD11c^highCD11b⁺ DCs from 4-week-old WT, perforin^−/−, DC-Fas^−/−, and perforin^−/−DC-Fas^−/− mice. (C) Flow cytometry of F4/80⁺CD11b⁺ macrophages in 4-week-old WT, perforin^−/−, DC-Fas^−/−, and perforin^−/−DC-Fas^−/− mice. (D) CD69 staining on CD4⁺, CD8⁺, and CD19⁺ cells from the spleen of 4-week-old WT, perforin^−/−, DC-Fas^−/−, and perforin^−/−DC-Fas^−/− mice were analyzed by flow cytometry. Splenocytes were also stained with APC–anti-CD4 or APC–anti-CD8, and PE–anti-CD44 and cychrome–anti-CD62L. The cells were analyzed by flow cytometry. CD44 and CD62L staining of CD4⁺ or CD8⁺ cells were plotted. Data are representative of 5 independent experiments.