Uncontrolled production of inflammatory cytokines and chemokines in 7-week-old perforin^−/−DC-Fas^−/− mice. (A) Sera from 7-week-old WT, perforin^−/− (Perf^−/−), DC-Fas^−/−, and Perf^−/−DC-Fas^−/− mice were used for measurement of different cytokines by multiplex. Statistic comparison between WT and Perf^−/−DC-Fas^−/−: for all cytokine except IL-12, P < .001; IL-12, not significant. (B) Percentages of F4/80⁺CD11b⁺ macrophages in mice as in panel A. P < .01 (n = 5). (C) Intracellular staining of TNF-α and IL-6 in F4/80⁺CD11b⁺ macrophages or CD11c⁺CD1b⁺ DCs from mice as in (A). Mean fluorescent intensity (MFI) for intracellular cytokine staining was plotted. **P < .01 (n = 5).