Figure 2
Figure 2. Platelet analyses in NMII-A mutant and control mouse blood. (Ai) Blood smear from an A+/A+ mouse shows normal size platelets (arrows), approximately one-tenth the diameter of erythrocytes. (Aii-vi) Giant platelets (arrows) with a size close to that of erythrocytes were easily detected in blood smears from all 3 mutant mouse lines. Blood smears are stained with Wright-Giemsa stain. (B) Mean platelet volumes (MPVs) of circulating platelets. MPVs from heterozygous and homozygous mutant mice are all significantly larger than the MPV from A+/A+ mice. Results represent mean ± SD. A+/A+ MPVs: 6.15 ± 0.45 fL (n = 18); AgfpR702C/A+: 9.13 ± 1.16 fL (n = 12); AD1424N/A+: 9.67 ± 1.44 fL (n = 10); AD1424N/AD1424N: 19.95 ± 6.45 fL (n = 4); AE1841K/A+: 11.37 ± 1.53 fL (n = 13); and AE1841K/AE1841K: 16.40 ± 3.75 fL (n = 3). *P < .001, 1-way ANOVA compared with A+/A+. (C) Platelet diameters measured from peripheral blood smears. Platelet diameters (mean ± SD) of A+/A+: 2.12 ± 0.45 μm (n = 193); AgfpR702C/A+: 2.67 ± 0.78 μm (n = 225); AD1424N/A+: 3.19 ± 0.93 μm (n = 110); AD1424N/AD1424N: 4.01 ± 1.09 μm (n = 114); AE1841K/A+: 3.41 ± 0.88 μm (n = 169); and AE1841K/AE1841K: 3.63 ± 1.01 μm (n = 158). The box plot shows median, upper and lower quartiles, and upper and lower 5 percentiles of each group. *P < .001 1-way ANOVA compared with A+/A+. Platelet diameter was measured on images of Wright-Giemsa–stained blood smears with the use of ImageJ (NIH). (D) Platelet counts from peripheral blood. Platelet counts from heterozygous NMII-A mutant mice are all significantly lower than those of A+/A+ mice, and homozygous mutant mice have even lower platelet counts. Results represent mean ± SD. Circulating platelet counts of A+/A+: 1056.29 ± 172.23 K/μL (n = 21); AgfpR702C/A+: 774.67 ± 88.02 K/μL (n = 12); AD1424N/A+: 801.90 ± 225.76 K/μL (n = 22); AD1424N/AD1424N: 395.40 ± 131.80 K/μL (n = 5); AE1841K/A+: 878.79 ± 202.31 K/μL (n = 14); and AE1841K/AE1841K: 635.33 ± 157.15 K/μL (n = 3). *P < .001, 1-way ANOVA compared with A+/A+. (E) Tail bleeding times. Mutant mice have a prolonged tail bleeding time compared with A+/A+ mice. Bar indicates mean of each group. (F) Clot retraction test. Representative image shows that the AgfpR702C/A+ platelet sample (in right tube) has reduced clot retraction compared with the A+/A+ sample (left tube). Image was taken 3 hours after stimulation.

Platelet analyses in NMII-A mutant and control mouse blood. (Ai) Blood smear from an A+/A+ mouse shows normal size platelets (arrows), approximately one-tenth the diameter of erythrocytes. (Aii-vi) Giant platelets (arrows) with a size close to that of erythrocytes were easily detected in blood smears from all 3 mutant mouse lines. Blood smears are stained with Wright-Giemsa stain. (B) Mean platelet volumes (MPVs) of circulating platelets. MPVs from heterozygous and homozygous mutant mice are all significantly larger than the MPV from A+/A+ mice. Results represent mean ± SD. A+/A+ MPVs: 6.15 ± 0.45 fL (n = 18); AgfpR702C/A+: 9.13 ± 1.16 fL (n = 12); AD1424N/A+: 9.67 ± 1.44 fL (n = 10); AD1424N/AD1424N: 19.95 ± 6.45 fL (n = 4); AE1841K/A+: 11.37 ± 1.53 fL (n = 13); and AE1841K/AE1841K: 16.40 ± 3.75 fL (n = 3). *P < .001, 1-way ANOVA compared with A+/A+. (C) Platelet diameters measured from peripheral blood smears. Platelet diameters (mean ± SD) of A+/A+: 2.12 ± 0.45 μm (n = 193); AgfpR702C/A+: 2.67 ± 0.78 μm (n = 225); AD1424N/A+: 3.19 ± 0.93 μm (n = 110); AD1424N/AD1424N: 4.01 ± 1.09 μm (n = 114); AE1841K/A+: 3.41 ± 0.88 μm (n = 169); and AE1841K/AE1841K: 3.63 ± 1.01 μm (n = 158). The box plot shows median, upper and lower quartiles, and upper and lower 5 percentiles of each group. *P < .001 1-way ANOVA compared with A+/A+. Platelet diameter was measured on images of Wright-Giemsa–stained blood smears with the use of ImageJ (NIH). (D) Platelet counts from peripheral blood. Platelet counts from heterozygous NMII-A mutant mice are all significantly lower than those of A+/A+ mice, and homozygous mutant mice have even lower platelet counts. Results represent mean ± SD. Circulating platelet counts of A+/A+: 1056.29 ± 172.23 K/μL (n = 21); AgfpR702C/A+: 774.67 ± 88.02 K/μL (n = 12); AD1424N/A+: 801.90 ± 225.76 K/μL (n = 22); AD1424N/AD1424N: 395.40 ± 131.80 K/μL (n = 5); AE1841K/A+: 878.79 ± 202.31 K/μL (n = 14); and AE1841K/AE1841K: 635.33 ± 157.15 K/μL (n = 3). *P < .001, 1-way ANOVA compared with A+/A+. (E) Tail bleeding times. Mutant mice have a prolonged tail bleeding time compared with A+/A+ mice. Bar indicates mean of each group. (F) Clot retraction test. Representative image shows that the AgfpR702C/A+ platelet sample (in right tube) has reduced clot retraction compared with the A+/A+ sample (left tube). Image was taken 3 hours after stimulation.

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