Neutrophil inclusion bodies in NMII-A mutant mice. (A) Representative images show inclusion bodies (arrows) that were found in neutrophils from mutant (Aii-vi) but not control (Ai) mouse blood smears. Blood smears are stained with Wright-Giemsa. (B) Live cell imaging of neutrophils (arrows) from Agfp/A+ and AgfpR702C/A+ mouse BM. The NMII-A in AgfpR702C/A+ neutrophils is more heterogeneous and punctate compared with NMII-A in the Agfp/A+ control. Green indicates GFP signal; white signal, bone collagen SHG; and white spots, Hoechst-stained nuclei. Bar, 20 μm. (C) Immunofluorescence confocal images of fixed neutrophils from A+/A+ and AD1424N/AD1424N mice. NMHCII-A (green) is distributed in the cytoplasm of the A+/A+ neutrophil. Mutant NMHCII-A D1424N aggregates into circular to oval-shaped cytoplasmic spots in AD1424N/AD1424N neutrophil. DAPI (blue) stains nuclei.
