Methylation level of VTRNA2-1 in hematopoietic cells. Methylation of the VTRNA2-1 promoter in HSCs, myeloid progenitors, and mature hematopoietic cells from normal donors. (A) Bisulfite sequencing of pooled CD34⁺ cells from random donors showing fully methylated and fully unmethylated alleles. (B) Results from pyrosequencing of sorted hematopoietic cells and myeloid progenitors from a donor with a biphasic methylation pattern. All cell types carry a methylation level ∼ 35%. MPP indicates multipotent progenitors; CMP, common myeloid progenitors; MEP, megakaryocyte-erythroid progenitors; GMP, granulocyte-macrophage progenitors; and BM MNC, BM mononuclear cells. (C) Bisulfite sequencing of a region −250 bp from the TSS reveals allele-specific methylation, in an individual carrying an A/G SNP.