TIR8 mRNA expression in mice. (A) Relative mRNA expression of TIR8 in CD19⁺ enriched splenocytes of TCL1^tg/tg (TCL1) and wild type (WT) mice of indicated age. The expression of TIR8 was normalized to mGAPDH and the results presented as ratio between normalized TIR8 expression in the target samples and the calibrator (mean value of 3 samples of total WT splenocytes). Data shown are average of 3 mice ± SEM. Statistical significance was analyzed by t test. (B) Relative mRNA expression of TIR8 in CD19⁺ enriched splenocytes (mean purity of CD19⁺ cells: 83% of which CD19⁺ CD5⁺ cells: 4%) and peritoneal cells (mean percentage CD19⁺ cells: 61% of which CD19⁺ CD5⁺ cells were 56%). Seven mice were analyzed: 5 animals at the age of 15 months and 2 animals at the age of 22 months. The expression of TIR8 was normalized to mGAPDH and the results are presented as ratio between normalized TIR8 expression in the target samples and the calibrator (mean value of 3 replicates of total WT splenocytes with 27% CD19⁺ cells and 4.5% CD19⁺ CD5⁺ cells). Data shown are average of 7 mice ± SEM. Statistical significance was analyzed by t test.