Premature CLL-like expansions and shorter life span in TCL1 transgenic mice lacking TIR8. (A) 8-13 months old TCL1^tg/tgTIR8^wt/wt mice (n = 6, mean age: 10 months ± 0), age-matched TCL1^tg/tgTIR8^−/− (n = 6, mean age: 10 months ± 1.8 as described in Table 1) and > 13 months old TCL1^tg/tgTIR8^wt/wt (n = 3, one 18 months, one 19 months and one 14 months old, respectively; mean age 17 months ± 2.6) mice were analyzed for accumulation of CD19⁺CD5⁺ B cells within different organs by flow cytometry. The mean value ± SD of the relative contribution of CD19⁺CD5⁺ cells to the whole B cell pool in the BM, SP, PE and PB, is shown. Statistical significance was analyzed by t test. (B) Kaplan-Meier survival curves of TCL1^tg/tgTIR8^wt/wt (n = 8) and TCL1^tg/tgTIR8^−/− (n = 7) mice. Statistical analysis between groups was performed using the log-rank test (median survival: 14 and 10 for TCL1^tg/tgTIR8^wt/wt and TCL1^tg/tgTIR8^−/− mice, respectively). Mice were included in the analysis after spontaneous death or when killed because of signs/symptoms of illness.