In vivo TLR-mediated downmodulation of CD5 expression on leukemic cells. (A) Leukemic TCL1^tg/tgTIR8^wt/wt mice were injected intraperitoneally either with 1μg/g LPS or PBS (n = 4 each group). 48 hours later, mice were bled and analyzed by flow cytometry. PB was analyzed before and after LPS injection for CD5 expression on leukemic cells. Plots from 1 mouse each group shown; 2 of 4 mice analyzed showed down-regulation of CD5 after LPS injection. MFI of CD19+CD5+ cells are indicated. (B) The same leukemic TCL1^tg/tgTIR8^wt/wt mice as in panel A were killed, organs removed and single-cell suspension analyzed by flow cytometry for the expression of CD19 and CD5. PE indicates peritoneal exudate; BM, bone marrow. Plots from 1 mouse in each group shown; 2 of 4 mice analyzed showed down-regulation of CD5 after LPS injection.