Figure 2
Figure 2. Granule membrane proteins display a limited codistribution with α-granule content proteins. (A-L) Qualitative comparisons of the distributions of VEGF versus CD40L (membrane protein, A-D), fibrinogen versus p-selectin (membrane protein, E-H), and fibrinogen versus VAMP8 (membrane protein, I-L). Images are maximum intensity projections of confocal image stacks. (M) Quantification of the limited codistribution. Microscope, Zeiss Axiovert 200M (Carl Zeiss); Objective lens, Zeiss planapochromat 100×/1.40NA oil objective, Imaging medium, buffered Mowiol containing 1% N-propylgallate as anti-fade reagent, Camera QImaging Retiga EXi (QImaging); Image acquisition software, iVision-Mac (Biovision Technologies) Version 4.0.16; Image deconvolution and colocalization software, Huygens Professional (Scientific Volume Imaging) Version 3.6. All pairings are quantified for a minimum of 30 individual platelets, averaged, and presented as the mean ± SEM.

Granule membrane proteins display a limited codistribution with α-granule content proteins. (A-L) Qualitative comparisons of the distributions of VEGF versus CD40L (membrane protein, A-D), fibrinogen versus p-selectin (membrane protein, E-H), and fibrinogen versus VAMP8 (membrane protein, I-L). Images are maximum intensity projections of confocal image stacks. (M) Quantification of the limited codistribution. Microscope, Zeiss Axiovert 200M (Carl Zeiss); Objective lens, Zeiss planapochromat 100×/1.40NA oil objective, Imaging medium, buffered Mowiol containing 1% N-propylgallate as anti-fade reagent, Camera QImaging Retiga EXi (QImaging); Image acquisition software, iVision-Mac (Biovision Technologies) Version 4.0.16; Image deconvolution and colocalization software, Huygens Professional (Scientific Volume Imaging) Version 3.6. All pairings are quantified for a minimum of 30 individual platelets, averaged, and presented as the mean ± SEM.

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