Increased expression of IFN-γ message in patients with T-LGL leukemia. TaqMan real-time RT-PCR analysis of IFN-γ mRNA levels in the PBMCs from T-LGL leukemia patients. The copy numbers of IFN-γ mRNA were normalized to the copy numbers of HPRT1 mRNA. The fold induction was calculated based on the normalized IFN-γ mRNA copy number in PBMCs from normal donors (n = 3).