Methylation and prognosis. Using a Bayes moderated t test, the most significant CpG sites for achieving CR after one induction (n = 42) and for 2-year survival (n = 62) were selected. Red represents more methylation; and green, less methylation. (A) Heat map showing a one-way hierarchical clustering analysis of CpG residues (columns) according to CR after first induction (rows). (B) Left panel: Two-way hierarchical clustering of CpG residues (columns) and samples (rows) according to the most significant residues for 2-year survival. Right panel: Kaplan-Meier diagrams demonstrate the discriminating ability of the selected CpG sites on OS. (C-D) Kaplan-Meier diagram showing the impact of the methylation levels of PcG-marked genes on OS and PFS. The samples were divided in tertiles according to the average methylation levels of PcG-marked genes. The tertile with most methylation (tertile 3), marked with a solid line, had significantly better OS (C) and PFS (D) than the less methylated tertiles; in the test cohort (top panels): P(trend) = .001 and .002, respectively. In the validation cohort (bottom panels): P(trend) = .009 and P(trend) = .035 (middle panels), and for pooled samples: P(trend) = .00009 and .0002, respectively.