CCL5 has no effect on cell migration, survival, and proliferation. (A) Migration ability of BCWM.1, HS-5, WMsc, and CD19⁺CD138⁺ from WM patient cells (WM cells) in response to CCL5 (500 ng/mL). Data represent fold-increased migration compared with either media alone or CCL5 as described. (B) Effect of CCL5 (500 ng/mL) on the viability of BCWM.1, HS-5, and CD19⁺CD138⁺ cells from WM patients (n = 3). Data represent cell viability in the presence of CCL5 compared with the media alone. (C) Effect of CCL5 on the proliferation of serum starved BCWM.1, HS-5, and CD19⁺CD138⁺ cells from WM patients (n = 3). Cell proliferation was assessed using thymidine incorporation and is presented as relative proliferation compared with cells cultured in the presence of media alone. (D) IgM secretion by BCWM.1 or CD19⁺CD138⁺ cells from WM patient cultured in the presence or absence of CCL5 (500 ng/mL). Values represent mean of duplicate values ± SEM.