Neutrophil recruitment is accelerated in Adam17-null hematopoietic chimeras in a model of sterile peritonitis. Neutrophil recruitment was analyzed in the sterile peritonitis model at different time points by evaluation of cell numbers using flow cytometry and an Ly6G antibody to specifically assess neutrophils. Circulating (A) and peritoneal (B) cells were collected (n = 5 per group). Data are representative of 4 experiments, including experiments at additional time points. * P = .02. (C) Mixed chimeras were generated containing 50% each WT (Ly5.1) and Adam17-null (Ly5.2) bone marrow. The relative contribution of Adam17^+/+ and Adam17^−/− neutrophils to total circulating (unstimulated, t = 0, n = 8) and peritoneal (4 hours after thioglycollate injection, n = 6) were evaluated. *P = .007 and **P = .01 for the change in neutrophil contribution from peritoneal to circulating. Mixed chimeras with different ratios of null and WT were evaluated in 2 other experiments, 1 at 2 hours and 1 at 4 hours after thioglycollate administration, with similar effects observed. All data are expressed as means ± SD.