RON is induced by EBV infection. (A) Total RNA was extracted from primary B cells and 3 LCLs and cDNA was synthesized using [³³P]–labeled PTK degenerate primers. RON-specific cDNAs could be digested by 3 restriction enzymes, MwoI, HaeIII, and RsaI. (B) The amounts of RON transcripts were detected by RT-PCR using specific RON primers. Detection of HPRT served as an internal control. (C) Total RNA and protein were harvested at the days post-EBV infection indicated. RON transcripts were detected by RT-Q-PCR and the relative fold increase was normalized to the amounts of RON transcripts in uninfected primary B cells (top panel). The expression of RON, EBNA1, LMP1, and β-actin proteins was measured by Western blotting (bottom panel). SE indicates short exposure; and LE, long exposure. (D) The expression of RON transcripts in various EBV-immortalized LCLs was detected by RT-Q-PCR and the relative fold increase of RON transcripts was normalized to the amounts of RON transcripts in uninfected primary B cells.