Reprogramming of EBV-B cells into iPSCs. (A-B) Diagram of the reprogramming process of AAT-deficient patient fibroblasts (A) and EBV-transformed B-cell lines (B). Bright-field images of parental cells before reprogramming and typical colonies right before colony picking are shown. (C-D) Immunofluorescence analysis of pluripotency markers for fibroblast-derived iPSCs (C, iAF3 is shown) and EBV-B cell–derived iPSCs (D, iAB5 is shown). Expression of PSC surface antigens TRA-1-60 and SSEA4 and transcription factors OCT4 and NANOG are observed (magnification, ×200). (E,I) Karyotype analyses for iAF3 and iAB5 lines, respectively. (F-H, J-L) Hematoxylin and eosin staining of teratomas derived from immunodeficient mice injected with either iAF3 (F-H) or iAB5 (J-L) show tissues representing all 3 germ layers (magnification, ×200). Ectoderm (F,J): PE indicates pigmented epithelium; NR, neural rosettes. Mesoderm (G,K): MC indicates mature cartilage; IC, immature cartilage. Endoderm (H,L): RGE indicates respiratory glandular epithelium; IGE, intestinal glandular epithelium.