Simvastatin inhibits 15(S)-HETE–induced Rac1 activation in HDMVECs and suppresses their migration and tube formation. (A,B) Quiescent HDMVECs were treated with and without 0.1μM 15(S)-HETE for the indicated time periods or 60 minutes in the presence and absence of 10μM simvastatin and Rac1 activation was measured by pull-down assay. Total cellular Rac1 levels were measured by Western blotting. (C,D) Quiescent HDMVECs that were treated with and without simvastatin were subjected to 15(S)-HETE–induced migration (C) or tube formation (D). The bar graphs represent the mean ± SD values of 3 independent experiments. *P < .01 versus control, **P < .01 versus 15(S)-HETE.