Lack of 12/15-Lox gene impairs blood flow recovery after ischemia. WT and 12/15-Lox^−/− mice were subjected to hind-limb ischemia by left femoral artery excision. (A) On day 7 after hind-limb ischemia, blood flow was measured by Laser Doppler Perfusion Imager (LDPI). Perfusion was expressed as ratio of the ischemic to the non-ischemic hind limb. (B) Blood vessels in the ischemic adductor muscles of WT and 12/15-Lox^−/− mice were analyzed by double immunofluorescence staining for CD31 (Red) and vWF (Green). (C) Ischemic and non-ischemic adductor muscle tissue extracts from WT and 12/15-Lox^−/− mice were prepared and analyzed for Rac1 fanesylation and activation. (D-E) RNA and protein extracts were prepared from ischemic and non-ischemic adductor muscles of WT and 12/15-Lox^−/− mice and analyzed for HMG-CoA reductase mRNA (D) and protein levels (E) by QRT-PCR and Western blotting, respectively, as described in Figure 3. (F) Ischemic and non-ischemic adductor muscle sections of WT and 12/15-Lox^−/− mice were stained for CD31 (green) and HMG-CoA reductase (red). The bar graphs in panels A, C, D and E represent the mean ± SD values of 3 independent experiments or 6 animals. *P < .05 versus WT non-ischemia; **P < .05 versus WT ischemia.