ATP, UTP, and BzATP trigger intracellular Ca²⁺ concentration increases in AML cells. Cells from AML patients were resuspended in a standard saline solution and loaded with the Ca²⁺ indicator Fura-2 AM, as reported in “Methods.” Cells were then rinsed and resuspended in the same saline solution. Stimulation was performed with 1mM ATP (A), 300μM BzATP (B), or 1mM UTP (C) on AML cells from the same patients. Closed triangles indicate nucleotide additions. One representative experiment is shown. Cells were also stimulated with increasing doses of ATP (D) or BzATP (E). Cells from the same patients were used for the ATP and BzATP stimulations.