Mean abnormal aneuploidy cell frequency (of a total 200 cells) and SD for the chromosome 7 probes ELN and D7S486/D7S522 scored together for loss to identify cells with monosomy 7), for chromosome 8q22 probe RUNX1T1 scored for gain to identify cells with trisomy 8 or tetrasomy 8, and for the chromosome 17p13 probe TP53 to identify cells with either loss (1 signal) or gain (3 or 4 signals) of TP53. The control samples are represented by the left bar at each time point and the G-CSF donor samples by the right bar at each time point. (A) Direct method. (B) Cultured method.