dnPPARγ overexpression causes a decrease in the T-cell population. (A) A representative flow cytometric analysis of CD4⁺ and CD8⁺ T cells from the BM, blood, spleens, and lungs from 3-month doxycycline-treated (+DOX) or untreated (−DOX) c-fms-rtTA/(tetO)₇-CMV-dnPPARγ–bitransgenic mice. (B) Percentage of CD4⁺ and CD8⁺ T cells in the BM, blood, spleens, and lungs of 3-month doxycycline-treated or untreated bitransgenic mice were statistically analyzed. (C) Total numbers of CD4⁺ and CD8⁺ T cells in the BM, spleens, and lungs of 3-month doxycycline-treated or untreated bitransgenic mice were statistically analyzed. (D) Representative flow cytometric analysis of CD25⁺Foxp3⁺ CD4⁺ Treg cells from the blood, spleens, and lungs of 3-month doxycycline-treated or untreated bitransgenic mice. (E) Percentages of CD25⁺Foxp3⁺ CD4⁺ Treg cells from the blood, spleens, and lungs of 3-month doxycycline-treated or untreated bitransgenic mice were statistically analyzed. (F) Frequencies of CD4⁻CD8⁻ double-negative (DN), CD4⁺CD8⁺ double-positive (DP), CD4⁺ single-positive (SP), and CD8⁺ SP thymocytes in 1-month doxycycline-treated or untreated bitransgenic mice. (G) Frequencies of DN1 (CD44⁺CD25⁻), DN2 (CD44⁺CD25⁺), DN3 (CD44⁻CD25⁺), and DN4 (CD44⁻CD25⁻) thymocytes in the same groups of mice. In all analyses, values were derived from 5 mice in each groups (n = 5). *P < .05; **P < .01.