Figure 6
Figure 6. c-Maf−/− fetal liver cells can reconstitute adult hematopoiesis in lethally irradiated mice. (A) Eight to 10 weeks after transplantation, the donor leukocyte chimerism of the mice reconstituted with c-Maf−/− fetal liver cells was comparable to that of the mice reconstituted with c-Maf+/+ fetal liver cells. The reconstitution efficiency was checked by flow cytometry with the use of the Ly5.1/Ly5.2 ratio of peripheral blood cells. Donor chimerism was determined to be as follows: (%Ly5.1+/%Ly5.1+ + %Ly5.2+) × 100. (B) No significant difference was found in the proportion of each lineage in peripheral blood between c-Maf+/+ fetal liver cells transplanted into mice and c-Maf−/− fetal liver cells transplanted into mice. (C) Flow cytometric analyses of the TER-119 and CD71 expression in total BM cells prepared from the femur of mice that received a transplant with c-Maf+/+ fetal liver cells (left) or c-Maf−/− fetal liver cells (right). The gates of CD71+/TER-119− (top left region: Q1), CD71+/TER-119+ (top right region: Q2), and CD71−/TER-119+ (bottom right region: Q3) in BM cells are defined as indicated. (D) Frequencies (%) of cells found in each region are shown. (E) Cell numbers of each region per femur are shown. (F) Flow cytometric analyses of the TER-119 and CD71 expression in total spleen cells prepared from mice that received a transplant with c-Maf+/+ fetal liver cells (left) and c-Maf−/− fetal liver cells (right). The frequencies (%) of CD71+ (top left and top right regions) and CD71−/TER-119+ (indicated squared gate in the bottom right region) cells in the spleen are indicated. (G) Frequencies (%) of each region are shown. (H) Cell numbers of each region per spleen are shown. Note that there are no significantly different frequencies or numbers of BM or spleen cells between mice that received a transplant with c-Maf+/+ fetal liver cells versus with c-Maf−/− fetal liver cells. □ represents mice that received a transplant with c-Maf+/+ fetal liver cells; ■, mice that received a transplant with c-Maf−/− fetal liver cells; n = 4 per group. FLC indicates, fetal liver cell.

c-Maf−/− fetal liver cells can reconstitute adult hematopoiesis in lethally irradiated mice. (A) Eight to 10 weeks after transplantation, the donor leukocyte chimerism of the mice reconstituted with c-Maf−/− fetal liver cells was comparable to that of the mice reconstituted with c-Maf+/+ fetal liver cells. The reconstitution efficiency was checked by flow cytometry with the use of the Ly5.1/Ly5.2 ratio of peripheral blood cells. Donor chimerism was determined to be as follows: (%Ly5.1+/%Ly5.1+ + %Ly5.2+) × 100. (B) No significant difference was found in the proportion of each lineage in peripheral blood between c-Maf+/+ fetal liver cells transplanted into mice and c-Maf−/− fetal liver cells transplanted into mice. (C) Flow cytometric analyses of the TER-119 and CD71 expression in total BM cells prepared from the femur of mice that received a transplant with c-Maf+/+ fetal liver cells (left) or c-Maf−/− fetal liver cells (right). The gates of CD71+/TER-119− (top left region: Q1), CD71+/TER-119+ (top right region: Q2), and CD71−/TER-119+ (bottom right region: Q3) in BM cells are defined as indicated. (D) Frequencies (%) of cells found in each region are shown. (E) Cell numbers of each region per femur are shown. (F) Flow cytometric analyses of the TER-119 and CD71 expression in total spleen cells prepared from mice that received a transplant with c-Maf+/+ fetal liver cells (left) and c-Maf−/− fetal liver cells (right). The frequencies (%) of CD71+ (top left and top right regions) and CD71−/TER-119+ (indicated squared gate in the bottom right region) cells in the spleen are indicated. (G) Frequencies (%) of each region are shown. (H) Cell numbers of each region per spleen are shown. Note that there are no significantly different frequencies or numbers of BM or spleen cells between mice that received a transplant with c-Maf+/+ fetal liver cells versus with c-Maf−/− fetal liver cells. □ represents mice that received a transplant with c-Maf+/+ fetal liver cells; ■, mice that received a transplant with c-Maf−/− fetal liver cells; n = 4 per group. FLC indicates, fetal liver cell.

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