Responses of mice that received a transplant with c-Maf^−/− fetal liver cells to induce anemia. (A) Comparisons of mRNA expression of Mac-1, c-Maf, VCAM-1, and Integrin αV are shown. Total RNA obtained from the Mac-1⁺ positive fraction (gray bar) and the Mac-1⁻ fraction (open bar) of BM cells was used for analyses. Note that VCAM-1 mRNA expression of the Mac-1⁺ fraction in mice that received a transplant with c-Maf^−/− fetal liver cells is comparable with that of control mice that received a transplant with c-Maf^+/+ fetal liver cells; n = 5 per group; FLC indicates fetal liver cell; Expression of the c-Maf^+/+ BM Mac-1 fraction was set to 1.0. All of the data are presented as mean ± SEM. (B) Mice with a baseline hematocrit of ≥ 35% were used. Four mice that received a transplant with c-Maf^+/+ fetal liver cells (open circle with solid line) and 4 mice that received a transplant with c-Maf^−/− fetal liver cells (open square with dashed line) were injected with phenylhydrazine on days 0, 1, and 3. Hematocrit levels were assessed on days 0, 3, 6, 8, and 10. Data are mean ± SEM.