WASp-deficient DCs fail to prime T cells. (A) Confocal analysis of MTOC localization, cells were stained for γ-tubulin (green) and TCR (red), asterisk indicates MTOC and scale bar represents 5 μm. Quantification of MTOC distance to the T-DC interface in (B) T cells and (C) DCs, data are of 4 independent experiments, C57BL/6 n = 28, WAS KO n = 32 and Y293F WASp n = 20 cells in total. (D) IL-12 production by DCs in coculture with OT-II T cells (representative plots shown from 1 experiment) was determined by intracellular FACS and quantified in panel E, showing average ± SEM, C57BL/6 n = 5, WAS KO n = 5, Y293F n = 3, P values are indicated (Student t test).