Structural model of talin-integrin interaction at the plasma membrane. Talin binds to 2 sites in the integrin-β3 tail: the membrane distal (MD) interaction site centered on NPxY⁷⁴⁷ motif and a membrane proximal (MP) site. Talin also makes contact with the plasma membrane at its interface with the cytosol (yellow band) through the positively charged residues on its surface (represented as blue; red dotted lines represent the membrane contact interfaces). Red and green represent the integrin-αIIb and β3 TM domains from the recent NMR structure (PDB entry 2k9j). Talin head domain (PDB entry 3IVF) is shown in surface representation. The ribbon representation of β3 was extended to the cytoplasmic domain by aligning the β3 TM domain with the β1D cytoplasmic domain structure from a β1D-Talin F2F3 (PDB entry 3G9W).