Diagnosing FHL3 by Western blotting using platelet protein. The amount of Munc13-4 protein expression was compared between platelets and PBMCs (A) and among platelets, CD8⁺, CD4⁺, CD19⁺, and CD14⁺ cells (B) by Western blotting. A representative result of 5 independent experiments is shown. (C) Six FHL3 patients were diagnosed by Western blotting for Munc13-4 protein using platelets.