Selection against the IGHV4-34 gene and long IGH-CDR3s in all 6 memory B-cell subsets. (A) Frequencies of the most commonly used IGHV3 genes in cloned IGH gene rearrangements. Differences between each memory B-cell subset compared with naive mature B cells were statistically analyzed with the χ2 test. (B) Frequencies of the most commonly used IGHV4 genes in cloned IGH gene rearrangements. An arrow indicates IGHV4-34 gene use in naive mature B cells. Differences between each memory B-cell subset compared with naive mature B cells were statistically analyzed with the χ2 test. *P < .05, **P < .01. (C) IGH-CDR3 size distributions. All individual sizes are indicated for each subset as gray dots, with red lines representing the median values. The dashed and dotted lines represent median values for centroblasts (n = 67) and centrocytes (n = 55), respectively. Differences between each memory B-cell subset compared with naive mature B cells were statistically analyzed with the Mann-Whitney test. **P < .01, ***P < .001.
