Impaired both IFN-γ and IFN-α responses in a STAT1-deficient patient. (A-B) PBMCs from the patient and a healthy subject were stimulated in vitro for 4 hours with IFN-α (10 000 U/mL), IFN-γ (100 ng/mL), IL-12 (25 ng/mL), or medium alone to determine c-Myc (A) and PIM-1 (B) expression. The experiments shown are representative of 4 independent experiments. Target gene expression was normalized to the housekeeping gene (β-2-microglobulin) and presented as n-fold increase of the expression over unstimulated cells from the healthy control. Data are mean ± SE. *Significant difference in the response between the patient and control subject (P < .05).