American Society of Hematology

Figure 1

$Figure 1. Zebrafish G-csf and Epo increase colony formation from unfractionated mpx:eGFP WKM. (A) CFUs/100 000 unfractionated WKM cells plated in methylcellulose with combinations of carp serum, G-csf, or Epo added. Bars represent average of at least 3 independent experiments, with error bars representing SD. Black bars represent Mpx:eGFP− colonies and green bars represent Mpx:eGFP+ colonies. (B) Brightfield (top rows) and GFP fluorescent images (bottom rows) of representative colonies enumerated in panel A. All images were taken at 50×. Scale bar in top left panel is 50 μm.$

Zebrafish G-csf and Epo increase colony formation from unfractionated mpx:eGFP WKM. (A) CFUs/100 000 unfractionated WKM cells plated in methylcellulose with combinations of carp serum, G-csf, or Epo added. Bars represent average of at least 3 independent experiments, with error bars representing SD. Black bars represent Mpx:eGFP⁻ colonies and green bars represent Mpx:eGFP⁺ colonies. (B) Brightfield (top rows) and GFP fluorescent images (bottom rows) of representative colonies enumerated in panel A. All images were taken at 50×. Scale bar in top left panel is 50 μm.

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