American Society of Hematology

Figure 2

$Figure 2. Precursor and lymphoid fractions of WKM contain myeloid and erythroid progenitors. (A) Experimental schematic for isolation and culture of mpx:eGFP−; gata1:DsRed− cells from precursor (blue) and lymphoid (purple) fraction of adult WKM. (B) CFUs/100 000 mpx:eGFP−, gata1:DsRed− precursor cells plated in methylcellulose with combinations of carp serum, G-csf, or Epo added. Red bars represent gata1:DsRed+ colonies, green bars represent mpx:eGFP+ colonies, and black bars represent negative colonies generated from the precursor fraction. (C) CFUs/100 000 mpx:eGFP−, gata1:DsRed− lymphoid cells plated in methylcellulose with combinations of carp serum, G-csf, or Epo added. Red bars represent gata1:DsRed+ colonies, green bars represent mpx:eGFP+ colonies, and black bars represent negative colonies generated from the lymphoid fraction. Bars represent average of at least 3 independent experiments, with error bars representing SD.$

Precursor and lymphoid fractions of WKM contain myeloid and erythroid progenitors. (A) Experimental schematic for isolation and culture of mpx:eGFP⁻; gata1:DsRed⁻ cells from precursor (blue) and lymphoid (purple) fraction of adult WKM. (B) CFUs/100 000 mpx:eGFP⁻, gata1:DsRed⁻ precursor cells plated in methylcellulose with combinations of carp serum, G-csf, or Epo added. Red bars represent gata1:DsRed⁺ colonies, green bars represent mpx:eGFP⁺ colonies, and black bars represent negative colonies generated from the precursor fraction. (C) CFUs/100 000 mpx:eGFP⁻, gata1:DsRed⁻ lymphoid cells plated in methylcellulose with combinations of carp serum, G-csf, or Epo added. Red bars represent gata1:DsRed⁺ colonies, green bars represent mpx:eGFP⁺ colonies, and black bars represent negative colonies generated from the lymphoid fraction. Bars represent average of at least 3 independent experiments, with error bars representing SD.

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