SHP2 knockdown reduces C/EBPα expression in 32Dcl3 cells. (A) Total cellular protein samples from from vector-transduced 32Dcl3 cells (Puro) and from subclones expressing SHP2 shRNA-77 were subjected to Western blotting for C/EBPα, PU.1, RUNX1, and β-actin. (B) Total cellular RNAs prepared from these same cell lines proliferating in IL-3 (left) or after transfer to G-CSF for one day (right) were converted to first-strand cDNA and the relative expression of CEBPA and PU.1 were analyzed by real-time PCR analysis. Shown are means and SE from 3 independent RNA preparations. * indicates P < .05 for each RNA level relative to expression in the vector-transduced cells.