Analysis of E-selectin ligand expression on human CD34⁺ cells and mouse LSK cells. (A) Representative flow cytometric histograms of E-Ig staining of mouse LSK cells and human CD34⁺ cells. (B) E-Ig was used to immunoprecipitate E-selectin ligands from cell lysates of human CD34⁺ cells and mouse LSK cells. SDS-PAGE–resolved material was subsequently immunoblotted with E-Ig.