CD43 on native human CD34⁺ and mouse LSK cells displays sialofucosylated O-glycans. CD43 was immunoprecipitated from membrane preparations of human CD34⁺ cells and mouse LSK cells, followed by treatment with enzymes as shown. Respective immunoprecipitates, normalized in each case for amount of input protein, were resolved on 4% to 20% SDS-PAGE, blotted, and stained with mAb HECA-452. Absence of staining after neuraminidase digestion confirms that ∼ 130 kDa CD43 is decorated with sialic acid. Abrogation of HECA-452 reactivity after OSGE digestion demonstrates that sialofucosylations of CD43 on respective human and mouse cells are displayed predominantly on O-linked glycans. Results are representative of at least 3 separate experiments.