Effect of AID-mediated genomic damage on tumorigenicity of cells in immune-deficient mice. Eight-week-old female NSG mice were injected intravenously with 1 × 10⁶ U266 cells per mouse, either U266 alone or re-isolated after coculture with DCs (U266 coculture) as in Figure 1A. Mice were killed when they had palpable tumors or showed other disease symptoms, including hunched posture, failure to eat and drink, and paralysis. (A) IHC analysis of tumor mass in a mouse injected with control tumor cells showing presence of CD138⁺ tumor cells. (B) Survival curve of mice injected with tumor cells alone or those previously exposed to DCs. (C) Detection of hCD138⁺ tumor cells in the spleen of mice injected with tumor cells previously exposed to DCs and PBS control. Bottom panel represents a higher magnification.